Composite

Part:BBa_K3830017:Design

Designed by: Fang-Ti   Group: iGEM21_NCHU_Taichung   (2021-10-18)


pGET-118 inserted with celK, T7 terminator and pr promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1841
    Illegal EcoRI site found at 1909
    Illegal XbaI site found at 116
    Illegal PstI site found at 699
    Illegal PstI site found at 1176
    Illegal PstI site found at 2061
    Illegal PstI site found at 2100
    Illegal PstI site found at 2466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1841
    Illegal EcoRI site found at 1909
    Illegal NheI site found at 2850
    Illegal PstI site found at 699
    Illegal PstI site found at 1176
    Illegal PstI site found at 2061
    Illegal PstI site found at 2100
    Illegal PstI site found at 2466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1841
    Illegal EcoRI site found at 1909
    Illegal BglII site found at 1
    Illegal BamHI site found at 1522
    Illegal XhoI site found at 467
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1841
    Illegal EcoRI site found at 1909
    Illegal XbaI site found at 116
    Illegal PstI site found at 699
    Illegal PstI site found at 1176
    Illegal PstI site found at 2061
    Illegal PstI site found at 2100
    Illegal PstI site found at 2466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1841
    Illegal EcoRI site found at 1909
    Illegal XbaI site found at 116
    Illegal PstI site found at 699
    Illegal PstI site found at 1176
    Illegal PstI site found at 2061
    Illegal PstI site found at 2100
    Illegal PstI site found at 2466
    Illegal AgeI site found at 1286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2126


Design Notes

--


Source

celK comes fromClostridium thermocellum<I> pr promoter comes from <I>bacteriophage λ<I> T7 terminator comes from <I>T7 bacteriophage<I>


===References===